@article{Marjan-2017-Assessing,
title = "Assessing recovery of in vitro steroid production in male rainbow darter ({\textless}i{\textgreater}Etheostoma caeruleum{\textless}/i{\textgreater}) in response to municipal wastewater treatment plant infrastructure changes",
author = "Marjan, Patricija and
Kraak, Glen J. Van Der and
MacLatchy, Deborah L. and
Fuzzen, Meghan and
Bragg, Leslie M. and
McMaster, Mark E. and
Tetreault, Gerald R. and
Servos, Mark R.",
journal = "Environmental Toxicology and Chemistry, Volume 37, Issue 2",
volume = "37",
number = "2",
year = "2017",
publisher = "Wiley",
url = "https://gwf-uwaterloo.github.io/gwf-publications/G17-26001",
doi = "10.1002/etc.3986",
pages = "501--514",
abstract = "The present study examined in vitro 11-ketotestosterone and testosterone production by the testes of rainbow darter (Etheostoma caeruleum) collected from selected reference sites and downstream of 2 municipal wastewater treatment plants (MWWTPs; Waterloo and Kitchener) on the central Grand River (Ontario, Canada), over a 6-yr period (2011-2016). The main objective was to investigate if infrastructure upgrades at the Kitchener MWWTP in 2012 resulted in a recovery of this response in the post-upgrade period (2013-2016). Two supporting studies showed that the fall season is appropriate for measuring in vitro sex steroid production because it provides stable detection of steroid patterns, and that the sample handling practiced in the present study did not introduce a bias. Infrastructure upgrades of the Kitchener MWWTP resulted in significant reductions in ammonia and estrogenicity. After the upgrades, 11-ketotestosterone production by MWWTP-exposed fish increased in 2013 and it continued to recover throughout the study period of 2014 through 2016, returning to levels measured in reference fish. Testosterone production was less sensitive and it lacked consistency. The Waterloo MWWTP underwent some minor upgrades but the level of ammonia and estrogenicity remained variable over time. The production of 11-ketotestosterone and testosterone in rainbow darter below the Waterloo MWWTP was variable and without a clear recovery pattern over the course of the present study. The results of the present study demonstrated that measuring production of sex steroids (especially 11-ketotestosterone) over multiple years can be relevant for assessing responses in fish to environmental changes such as those resulting from major infrastructure upgrades. Environ Toxicol Chem 2018;37:501-514. {\copyright} 2017 SETAC.",
}
<?xml version="1.0" encoding="UTF-8"?>
<modsCollection xmlns="http://www.loc.gov/mods/v3">
<mods ID="Marjan-2017-Assessing">
<titleInfo>
<title>Assessing recovery of in vitro steroid production in male rainbow darter (\textlessi\textgreaterEtheostoma caeruleum\textless/i\textgreater) in response to municipal wastewater treatment plant infrastructure changes</title>
</titleInfo>
<name type="personal">
<namePart type="given">Patricija</namePart>
<namePart type="family">Marjan</namePart>
<role>
<roleTerm authority="marcrelator" type="text">author</roleTerm>
</role>
</name>
<name type="personal">
<namePart type="given">Glen</namePart>
<namePart type="given">J</namePart>
<namePart type="given">Van</namePart>
<namePart type="given">Der</namePart>
<namePart type="family">Kraak</namePart>
<role>
<roleTerm authority="marcrelator" type="text">author</roleTerm>
</role>
</name>
<name type="personal">
<namePart type="given">Deborah</namePart>
<namePart type="given">L</namePart>
<namePart type="family">MacLatchy</namePart>
<role>
<roleTerm authority="marcrelator" type="text">author</roleTerm>
</role>
</name>
<name type="personal">
<namePart type="given">Meghan</namePart>
<namePart type="family">Fuzzen</namePart>
<role>
<roleTerm authority="marcrelator" type="text">author</roleTerm>
</role>
</name>
<name type="personal">
<namePart type="given">Leslie</namePart>
<namePart type="given">M</namePart>
<namePart type="family">Bragg</namePart>
<role>
<roleTerm authority="marcrelator" type="text">author</roleTerm>
</role>
</name>
<name type="personal">
<namePart type="given">Mark</namePart>
<namePart type="given">E</namePart>
<namePart type="family">McMaster</namePart>
<role>
<roleTerm authority="marcrelator" type="text">author</roleTerm>
</role>
</name>
<name type="personal">
<namePart type="given">Gerald</namePart>
<namePart type="given">R</namePart>
<namePart type="family">Tetreault</namePart>
<role>
<roleTerm authority="marcrelator" type="text">author</roleTerm>
</role>
</name>
<name type="personal">
<namePart type="given">Mark</namePart>
<namePart type="given">R</namePart>
<namePart type="family">Servos</namePart>
<role>
<roleTerm authority="marcrelator" type="text">author</roleTerm>
</role>
</name>
<originInfo>
<dateIssued>2017</dateIssued>
</originInfo>
<typeOfResource>text</typeOfResource>
<genre authority="bibutilsgt">journal article</genre>
<relatedItem type="host">
<titleInfo>
<title>Environmental Toxicology and Chemistry, Volume 37, Issue 2</title>
</titleInfo>
<originInfo>
<issuance>continuing</issuance>
<publisher>Wiley</publisher>
</originInfo>
<genre authority="marcgt">periodical</genre>
<genre authority="bibutilsgt">academic journal</genre>
</relatedItem>
<abstract>The present study examined in vitro 11-ketotestosterone and testosterone production by the testes of rainbow darter (Etheostoma caeruleum) collected from selected reference sites and downstream of 2 municipal wastewater treatment plants (MWWTPs; Waterloo and Kitchener) on the central Grand River (Ontario, Canada), over a 6-yr period (2011-2016). The main objective was to investigate if infrastructure upgrades at the Kitchener MWWTP in 2012 resulted in a recovery of this response in the post-upgrade period (2013-2016). Two supporting studies showed that the fall season is appropriate for measuring in vitro sex steroid production because it provides stable detection of steroid patterns, and that the sample handling practiced in the present study did not introduce a bias. Infrastructure upgrades of the Kitchener MWWTP resulted in significant reductions in ammonia and estrogenicity. After the upgrades, 11-ketotestosterone production by MWWTP-exposed fish increased in 2013 and it continued to recover throughout the study period of 2014 through 2016, returning to levels measured in reference fish. Testosterone production was less sensitive and it lacked consistency. The Waterloo MWWTP underwent some minor upgrades but the level of ammonia and estrogenicity remained variable over time. The production of 11-ketotestosterone and testosterone in rainbow darter below the Waterloo MWWTP was variable and without a clear recovery pattern over the course of the present study. The results of the present study demonstrated that measuring production of sex steroids (especially 11-ketotestosterone) over multiple years can be relevant for assessing responses in fish to environmental changes such as those resulting from major infrastructure upgrades. Environ Toxicol Chem 2018;37:501-514. \copyright 2017 SETAC.</abstract>
<identifier type="citekey">Marjan-2017-Assessing</identifier>
<identifier type="doi">10.1002/etc.3986</identifier>
<location>
<url>https://gwf-uwaterloo.github.io/gwf-publications/G17-26001</url>
</location>
<part>
<date>2017</date>
<detail type="volume"><number>37</number></detail>
<detail type="issue"><number>2</number></detail>
<extent unit="page">
<start>501</start>
<end>514</end>
</extent>
</part>
</mods>
</modsCollection>
%0 Journal Article
%T Assessing recovery of in vitro steroid production in male rainbow darter (\textlessi\textgreaterEtheostoma caeruleum\textless/i\textgreater) in response to municipal wastewater treatment plant infrastructure changes
%A Marjan, Patricija
%A Kraak, Glen J. Van Der
%A MacLatchy, Deborah L.
%A Fuzzen, Meghan
%A Bragg, Leslie M.
%A McMaster, Mark E.
%A Tetreault, Gerald R.
%A Servos, Mark R.
%J Environmental Toxicology and Chemistry, Volume 37, Issue 2
%D 2017
%V 37
%N 2
%I Wiley
%F Marjan-2017-Assessing
%X The present study examined in vitro 11-ketotestosterone and testosterone production by the testes of rainbow darter (Etheostoma caeruleum) collected from selected reference sites and downstream of 2 municipal wastewater treatment plants (MWWTPs; Waterloo and Kitchener) on the central Grand River (Ontario, Canada), over a 6-yr period (2011-2016). The main objective was to investigate if infrastructure upgrades at the Kitchener MWWTP in 2012 resulted in a recovery of this response in the post-upgrade period (2013-2016). Two supporting studies showed that the fall season is appropriate for measuring in vitro sex steroid production because it provides stable detection of steroid patterns, and that the sample handling practiced in the present study did not introduce a bias. Infrastructure upgrades of the Kitchener MWWTP resulted in significant reductions in ammonia and estrogenicity. After the upgrades, 11-ketotestosterone production by MWWTP-exposed fish increased in 2013 and it continued to recover throughout the study period of 2014 through 2016, returning to levels measured in reference fish. Testosterone production was less sensitive and it lacked consistency. The Waterloo MWWTP underwent some minor upgrades but the level of ammonia and estrogenicity remained variable over time. The production of 11-ketotestosterone and testosterone in rainbow darter below the Waterloo MWWTP was variable and without a clear recovery pattern over the course of the present study. The results of the present study demonstrated that measuring production of sex steroids (especially 11-ketotestosterone) over multiple years can be relevant for assessing responses in fish to environmental changes such as those resulting from major infrastructure upgrades. Environ Toxicol Chem 2018;37:501-514. \copyright 2017 SETAC.
%R 10.1002/etc.3986
%U https://gwf-uwaterloo.github.io/gwf-publications/G17-26001
%U https://doi.org/10.1002/etc.3986
%P 501-514
Markdown (Informal)
[Assessing recovery of in vitro steroid production in male rainbow darter (<i>Etheostoma caeruleum</i>) in response to municipal wastewater treatment plant infrastructure changes](https://gwf-uwaterloo.github.io/gwf-publications/G17-26001) (Marjan et al., GWF 2017)
ACL
- Patricija Marjan, Glen J. Van Der Kraak, Deborah L. MacLatchy, Meghan Fuzzen, Leslie M. Bragg, Mark E. McMaster, Gerald R. Tetreault, and Mark R. Servos. 2017. Assessing recovery of in vitro steroid production in male rainbow darter (Etheostoma caeruleum) in response to municipal wastewater treatment plant infrastructure changes. Environmental Toxicology and Chemistry, Volume 37, Issue 2, 37(2):501–514.
