Environmental DNA of preservative ethanol performed better than water samples in detecting macroinvertebrate diversity using metabarcoding
Yu Wang, Kai Chen, Jin Gao, Meng Wang, Jie Dong, Yuwei Xie, John P. Giesy, Xiaowei Jin, Beixin Wang
Abstract
High‐throughput pipelines supported by eDNA metabarcoding have been applied in various freshwater ecosystems. Both eDNA in ethanol (EtOH) samples (ES‐eDNA) and in water samples (WS‐eDNA) can provide comprehensive classification lists with good taxonomic resolution and coverage for determining freshwater biodiversity and biomonitoring. But, the advantages of ES‐eDNA metabarcoding over WS‐eDNA metabarcoding remain unclear for routine assessments of diversity of benthic macroinvertebrates in streams.- Cite:
- Yu Wang, Kai Chen, Jin Gao, Meng Wang, Jie Dong, Yuwei Xie, John P. Giesy, Xiaowei Jin, and Beixin Wang. 2021. Environmental DNA of preservative ethanol performed better than water samples in detecting macroinvertebrate diversity using metabarcoding. Diversity and Distributions, Volume 27, Issue 10, 27(10):1989–2002.
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@article{Wang-2021-Environmental,
title = "Environmental DNA of preservative ethanol performed better than water samples in detecting macroinvertebrate diversity using metabarcoding",
author = "Wang, Yu and
Chen, Kai and
Gao, Jin and
Wang, Meng and
Dong, Jie and
Xie, Yuwei and
Giesy, John P. and
Jin, Xiaowei and
Wang, Beixin",
journal = "Diversity and Distributions, Volume 27, Issue 10",
volume = "27",
number = "10",
year = "2021",
publisher = "Wiley",
url = "https://gwf-uwaterloo.github.io/gwf-publications/G21-45001",
doi = "10.1111/ddi.13284",
pages = "1989--2002",
abstract = "High‐throughput pipelines supported by eDNA metabarcoding have been applied in various freshwater ecosystems. Both eDNA in ethanol (EtOH) samples (ES‐eDNA) and in water samples (WS‐eDNA) can provide comprehensive classification lists with good taxonomic resolution and coverage for determining freshwater biodiversity and biomonitoring. But, the advantages of ES‐eDNA metabarcoding over WS‐eDNA metabarcoding remain unclear for routine assessments of diversity of benthic macroinvertebrates in streams.",
}
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<abstract>High‐throughput pipelines supported by eDNA metabarcoding have been applied in various freshwater ecosystems. Both eDNA in ethanol (EtOH) samples (ES‐eDNA) and in water samples (WS‐eDNA) can provide comprehensive classification lists with good taxonomic resolution and coverage for determining freshwater biodiversity and biomonitoring. But, the advantages of ES‐eDNA metabarcoding over WS‐eDNA metabarcoding remain unclear for routine assessments of diversity of benthic macroinvertebrates in streams.</abstract>
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%0 Journal Article %T Environmental DNA of preservative ethanol performed better than water samples in detecting macroinvertebrate diversity using metabarcoding %A Wang, Yu %A Chen, Kai %A Gao, Jin %A Wang, Meng %A Dong, Jie %A Xie, Yuwei %A Giesy, John P. %A Jin, Xiaowei %A Wang, Beixin %J Diversity and Distributions, Volume 27, Issue 10 %D 2021 %V 27 %N 10 %I Wiley %F Wang-2021-Environmental %X High‐throughput pipelines supported by eDNA metabarcoding have been applied in various freshwater ecosystems. Both eDNA in ethanol (EtOH) samples (ES‐eDNA) and in water samples (WS‐eDNA) can provide comprehensive classification lists with good taxonomic resolution and coverage for determining freshwater biodiversity and biomonitoring. But, the advantages of ES‐eDNA metabarcoding over WS‐eDNA metabarcoding remain unclear for routine assessments of diversity of benthic macroinvertebrates in streams. %R 10.1111/ddi.13284 %U https://gwf-uwaterloo.github.io/gwf-publications/G21-45001 %U https://doi.org/10.1111/ddi.13284 %P 1989-2002
Markdown (Informal)
[Environmental DNA of preservative ethanol performed better than water samples in detecting macroinvertebrate diversity using metabarcoding](https://gwf-uwaterloo.github.io/gwf-publications/G21-45001) (Wang et al., GWF 2021)
- Environmental DNA of preservative ethanol performed better than water samples in detecting macroinvertebrate diversity using metabarcoding (Wang et al., GWF 2021)
ACL
- Yu Wang, Kai Chen, Jin Gao, Meng Wang, Jie Dong, Yuwei Xie, John P. Giesy, Xiaowei Jin, and Beixin Wang. 2021. Environmental DNA of preservative ethanol performed better than water samples in detecting macroinvertebrate diversity using metabarcoding. Diversity and Distributions, Volume 27, Issue 10, 27(10):1989–2002.