Metagenomics of Wastewater Influent from Wastewater Treatment Facilities across Ontario in the Era of Emerging SARS-CoV-2 Variants of Concern

Opeyemi U Lawal, Linkang Zhang, Valeria R. Parreira, R. Stephen Brown, Charles Chettleburgh, Nora Dannah, Robert Delatolla, Kimberly A. Gilbride, Tyson E. Graber, Golam Islam, James Knockleby, Siying Ma, Hanlan McDougall, R. Michael L. McKay, Aleksandra Mloszewska, Claire J. Oswald, Mark R. Servos, Megan Swinwood-Sky, Gustavo Ybazeta, Marc Habash, Lawrence Goodridge


Abstract
We report metagenomic sequencing analyses of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in composite wastewater influent from 10 regions in Ontario, Canada, during the transition between Delta and Omicron variants of concern. The Delta and Omicron BA.1/BA.1.1 and BA.2-defining mutations occurring in various frequencies were reported in the consensus and subconsensus sequences of the composite samples.
Cite:
Opeyemi U Lawal, Linkang Zhang, Valeria R. Parreira, R. Stephen Brown, Charles Chettleburgh, Nora Dannah, Robert Delatolla, Kimberly A. Gilbride, Tyson E. Graber, Golam Islam, James Knockleby, Siying Ma, Hanlan McDougall, R. Michael L. McKay, Aleksandra Mloszewska, Claire J. Oswald, Mark R. Servos, Megan Swinwood-Sky, Gustavo Ybazeta, et al.. 2022. Metagenomics of Wastewater Influent from Wastewater Treatment Facilities across Ontario in the Era of Emerging SARS-CoV-2 Variants of Concern. Microbiology Resource Announcements, Volume 11, Issue 7, 11(7).
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