@article{Huang-2023-Simultaneous,
title = "Simultaneous Detection of L‐Lactate and D‐Glucose Using DNA Aptamers in Human Blood Serum**",
author = "Huang, Po‐Jung Jimmy and
Liu, Juewen",
journal = "Angewandte Chemie International Edition, Volume 62, Issue 12",
volume = "62",
number = "12",
year = "2023",
publisher = "Wiley",
url = "https://gwf-uwaterloo.github.io/gwf-publications/G23-37001",
doi = "10.1002/anie.202212879",
abstract = "L-lactate is a key metabolite indicative of physiological states, glycolysis pathways, and various diseases such as sepsis, heart attack, lactate acidosis, and cancer. Detection of lactate has been relying on a few enzymes that need additional oxidants. In this work, DNA aptamers for L-lactate were obtained using a library-immobilization selection method and the highest affinity aptamer reached a Kd of 0.43 mM as determined using isothermal titration calorimetry. The aptamers showed up to 50-fold selectivity for L-lactate over D-lactate and had little responses to other closely related analogs such as pyruvate or 3-hydroxybutyrate. A fluorescent biosensor based on the strand displacement method showed a limit of detection of 0.55 mM L-lactate, and the sensor worked in 90 {\%} serum. Simultaneous detection of L-lactate and D-glucose in the same solution was achieved. This work has broadened the scope of aptamers to simple metabolites and provided a useful probe for continuous and multiplexed monitoring.",
}
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<abstract>L-lactate is a key metabolite indicative of physiological states, glycolysis pathways, and various diseases such as sepsis, heart attack, lactate acidosis, and cancer. Detection of lactate has been relying on a few enzymes that need additional oxidants. In this work, DNA aptamers for L-lactate were obtained using a library-immobilization selection method and the highest affinity aptamer reached a Kd of 0.43 mM as determined using isothermal titration calorimetry. The aptamers showed up to 50-fold selectivity for L-lactate over D-lactate and had little responses to other closely related analogs such as pyruvate or 3-hydroxybutyrate. A fluorescent biosensor based on the strand displacement method showed a limit of detection of 0.55 mM L-lactate, and the sensor worked in 90 % serum. Simultaneous detection of L-lactate and D-glucose in the same solution was achieved. This work has broadened the scope of aptamers to simple metabolites and provided a useful probe for continuous and multiplexed monitoring.</abstract>
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%0 Journal Article
%T Simultaneous Detection of L‐Lactate and D‐Glucose Using DNA Aptamers in Human Blood Serum**
%A Huang, Po‐Jung Jimmy
%A Liu, Juewen
%J Angewandte Chemie International Edition, Volume 62, Issue 12
%D 2023
%V 62
%N 12
%I Wiley
%F Huang-2023-Simultaneous
%X L-lactate is a key metabolite indicative of physiological states, glycolysis pathways, and various diseases such as sepsis, heart attack, lactate acidosis, and cancer. Detection of lactate has been relying on a few enzymes that need additional oxidants. In this work, DNA aptamers for L-lactate were obtained using a library-immobilization selection method and the highest affinity aptamer reached a Kd of 0.43 mM as determined using isothermal titration calorimetry. The aptamers showed up to 50-fold selectivity for L-lactate over D-lactate and had little responses to other closely related analogs such as pyruvate or 3-hydroxybutyrate. A fluorescent biosensor based on the strand displacement method showed a limit of detection of 0.55 mM L-lactate, and the sensor worked in 90 % serum. Simultaneous detection of L-lactate and D-glucose in the same solution was achieved. This work has broadened the scope of aptamers to simple metabolites and provided a useful probe for continuous and multiplexed monitoring.
%R 10.1002/anie.202212879
%U https://gwf-uwaterloo.github.io/gwf-publications/G23-37001
%U https://doi.org/10.1002/anie.202212879
Markdown (Informal)
[Simultaneous Detection of L‐Lactate and D‐Glucose Using DNA Aptamers in Human Blood Serum**](https://gwf-uwaterloo.github.io/gwf-publications/G23-37001) (Huang & Liu, GWF 2023)
ACL
- Po‐Jung Jimmy Huang and Juewen Liu. 2023. Simultaneous Detection of L‐Lactate and D‐Glucose Using DNA Aptamers in Human Blood Serum**. Angewandte Chemie International Edition, Volume 62, Issue 12, 62(12).
