Micromachines, Volume 12, Issue 4


Anthology ID:
G21-123
Month:
Year:
2021
Address:
Venue:
GWF
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Publisher:
MDPI AG
URL:
https://gwf-uwaterloo.github.io/gwf-publications/G21-123
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A Microflow Cytometry-Based Agglutination Immunoassay for Point-of-Care Quantitative Detection of SARS-CoV-2 IgM and IgG
Jianxi Qu | Mathieu Chenier | Yushan Zhang | Chang–Qing Xu

A rapid, sensitive and simple microflow cytometry-based agglutination immunoassay (MCIA) was developed for point-of-care (POC) quantitative detection of SARS-CoV-2 IgM and IgG antibodies. The antibody concentration was determined by using the transit time of beads aggregates. A linear relationship was established between the average transit time and the concentration of SARS-CoV-2 IgM and IgG, respectively. The limit of detection (LOD) of SARS-CoV-2 IgM and IgG by the MCIA measurement are 0.06 mg/L and 0.10 mg/L, respectively. The 10 µL sample consumption, 30 min assay time and the compact setup make this technique suitable for POC quantitative detection of SARS-CoV-2 antibodies.